Sequencing and Genotyping Facility
Capillary electrophoresis service
Clients perform their BDV3.1 cycle sequencing reactions (fluorescent labelling) and submit the dried extension product in a 1.5mL tube clearly labelled with sample ID for electrophoresis. We also require client details (name, lab/department, email and billing information) and PCR product size (if applicable) with each sample submission.
Turnaround Time
1 day from sample receipt. (Samples are required before 4pm for next day results, though we do operate on a first-in-best-dressed basis).
For plate submission we can provide you with a 96 well template.
Reaction and electrophoresis service
Clients supply a sample of their purified DNA template and primer in separate 1.5mL tubes. Each sample must be clearly labelled with sample ID and be accompanied with details of DNA concentration (ng/ul) and primer concentration (uM).
NOTE: It is imperative that the customer provides information regarding template size and type (i.e. PCR product, plasmid) as this will determine the amount of DNA we require to set up each sequence reaction. Generally we use 100ng per kilobase of DNA. Please also note that when determining the amount of DNA required, the entire plasmid size must be taken into account and not just the insert.
We require at least 2ul of Primer at a concentration of 5uM per reaction.
Please note that we set up 20ul reactions and therefore the total template volume should not exceed 10ul.
Turnaround Time
1 day from sample receipt. (Samples are required before 11am for next day results, though we do operate on a first-in-best-dressed basis).
Fragment Analysis / Genotyping
AGD also offers a fragment analysis service, involving the capillary electrophoresis of fluorescently-tagged PCR products. Please contact us for more information of acceptable labels, fragment sizes and for pricing information.